Type II Restriction Enzyme PvuII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-40   GLN-41  4.3 4.9 -7.7 -5.8 5.3 7.2 90.0
 GLN-41   VAL-42  2.9 3.2 -0.6 0.8 65.4 63.0 -18.1
 VAL-42   LEU-43  5.5 5.8 -0.1 2.1 95.7 91.6 -10.5

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees