Poly(a) Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 TYR-421   GLU-422  1.2 3.7 -16.8 -9.5 91.5 96.9 3.4
 GLU-422   MET-423  4.6 7.4 92.2 -86.7 92.1 114.6 1.6
 MET-423   ILE-424  5.0 7.5 -18.9 5.0 149.2 130.8 -13.9
 ILE-424   GLN-425  4.6 6.4 39.9 -55.2 52.6 35.1 -7.1
 GLN-425   ASP-426  6.1 8.2 59.6 -39.9 105.7 106.3 7.1
 ASP-426   LYS-427  9.1 11.5 22.4 61.2 76.9 80.0 23.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-459   TYR-460  4.0 1.4 -6.3 -11.2 35.6 36.5 19.7
 TYR-460   LEU-461  3.5 1.3 6.9 -20.2 103.5 110.2 8.5
 LEU-461   SER-462  2.6 3.1 -5.1 16.7 14.9 34.4 -25.1
 SER-462   THR-463  3.0 4.2 5.8 6.0 94.2 98.3 2.8
 THR-463   MET-464  6.2 7.8 12.3 -26.9 155.5 158.8 14.4
 MET-464   TYR-465  7.8 9.5 6.4 -6.9 79.2 92.4 -5.5
 TYR-465   ILE-466  11.6 13.1 12.9 -11.1 144.4 152.5 -5.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees