DynDom - Protein Domain Motions

T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Run Details Domains Sequence Morph Domain Pairs Contact Graph

DynDom run details

Property	Value
Name	T7 RNA Polymerase
Conformer 1 (PDB)	1cez (A)
Conformer 2 (PDB)	1aro (P)
Window Length	9
Minimum ratio	1.0
Minimum domain size	20

Domains

Domain	Size	Backbone RMSD (A)	Residues
1	613	1.94	12 - 554 710 - 710 712 - 848 853 - 855 864 - 871
2	141	2.54	555 - 709 711 - 711 849 - 852 861 - 863

Sequence

`________________________________________________________________________________________________________________________` `1cez(A) : IAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFp---LITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVA` `:` `1aro(P) : KNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLliTTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVA` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `1cez(A) : SAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRA` `:` `1aro(P) : SAIGRAIEDEARFGRIRDLEAKHFKK-----------------fMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQN-------sETIELAPEYAEAIATRA` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `1cez(A) : GALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWK` `:` `1aro(P) : GALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKW----------------------------------` `________________________________________________________________________________________________________________________` `________________________________________________________________________________________________________________________` `1cez(A) :` `RAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLE` `:` `1aro(P) :` `-----vYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLE` `________________________________________________________________________________________________________________________` `_____________________________________________________554________________________________________________________________` `1cez(A) : NTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAG` `:` `1aro(P) : NTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAING----------------------gTKALAG` `_____________________________________________________554________________________________________________________________` `________________________________________________________________________________________709_____________________________` `1cez(A) : QWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQE` `:` `1aro(P) : QWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRSAVHWVTPDGFPVWQE` `________________________________________________________________________________________709_____________________________` `___________________________________________________________________________________________________________848_852______` `1cez(A) : YKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQL` `:` `1aro(P) : YKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESSDVLADFYDQFADQLHESQL` `___________________________________________________________________________________________________________848_852______` `__863____________________` `1cez(A) : DKMPALPAKGNLNLRDILESDFAFA` `:` `1aro(P) : DKMPALPAKGNLNLRDILESD**` `__863____________________`

Morph

This morph was created using the MorphIt_Pro protein morphing technique.
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Domain Pairs

Property	Value
Fixed Domain ( blue )	1
Moving Domain ( red )	2
Rotation Angle (deg)	11.8
Translation (A)	-0.2
Closure (%)	99.3
Bending Residues ( green )	554 - 555 709 - 722 848 - 849 852 - 861 863 - 864
Bending Region Analysis

Dynamic Contact Graph

Conformer 1 Contact:
Residue 1 ———› Residue 2

Conformer 2 Contact:
Residue 2 ———› Residue 1

Movement classification: Shear

PyMOL Script

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